General buffers and salt solutions
In this section:
- HEPES 1 M, pH 7.4
- 1M MgCl2
- 10x Phosphate Buffered Saline (PBS)
- 20x Phosphate Buffered Saline (PBS)
- PBT
- Proteinase K(PK), 10 mg/ml
- PTw
- 4M NaCl, 1 liter
- SDS 10%
- TAE 50X
- 1M Tris pH 9.5
HEPES 1 M, pH 7.4
For 1Liter:
- Hepes 238.31 g
Dissolve, Adjust pH and volume. Store at RT.'
1M MgCl2
For 500 ml:
Dissolve 47.606 g MgCl2 in dH2O. Adjust volume, store at RT.
10x Phosphate Buffered Saline (PBS)
10X For 4 L bottle:
- NaCl 320 g
- KCl 8 g Na2HPO4 57.6 g
- KH2PO4 9.6 g
Dissolve in dH2O. Adjust pH to 7.4, and bring to volume. Store at RT.
20x PBS (phosphate-buffered saline)
- Na2PO4 H20 - 5.12 g
"Ha2HPO4 - 23.88 g
- NaCl - 204.4 g
- distilled water - 800 ml
Adjust pH to 7.4 and volume to 1 liter.
PBT
- PBS (phosphate buffered saline)
- 2 mg/ml BSA (bovine serum albumin) (Fraction 5, reagent grade)
- 0.01% Triton X-100
Proteinase K(PK), 10 mg/ml
Supplied as 100 mg powder, (American Bioanalytical AB00925-000100)
Add 10 ml ddH20 for conc. of 10 mg/ml, mix until dissolved. Divide into 150 µl aliquots, store in -20C
PTw
1X PBS(phosphate buffered saline)
0.1% Tween-20
4M NaCl, 1 liter
Dissolve 233.76 g NaCl in 750 ml dH2O. Adjust volume.
SDS 10%
For 1 L:
Dissolve 100 g SDS in 800 ml of dH2O. Adjust volume, store at RT.
Sodium Phosphate Buffer(1M)
- 71 g Na2Hpo4
- 4 ml H3P04
- 1 Liter H20 titrate to pH 7.4
TAE 50X
For 2 L:
- Tris 484 g
- 114.2 ml Glacial Acetic acid
- 200 ml 0.5 M EDTA pH 8.0
Dissolve in dH2O, adjust volume. Store at RT.
1M Tris pH 9.5
For 1 L:
Dissolve 121.14 g Tris in 700 ml dH2O. Adjust pH to 9.5 with HCl Adjust volume, store at RT.